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Journal: Redox Report : Communications in Free Radical Research
Article Title: Coordinated mitochondrial quality control underlies oxidative stress resilience in MUSE stem cells
doi: 10.1080/13510002.2026.2698189
Figure Lengend Snippet: MUSE cells preserve mitochondrial respiratory function and membrane potential under oxidative stress (A) Mitochondrial respiratory activity was evaluated 24 h after H₂O₂ exposure using a fluorescence-based oxygen-consumption assay. OCR profiles were obtained following sequential treatment with oligomycin, FCCP, and rotenone. Histograms summarize basal respiration, ATP-linked respiration, maximal respiration, and spare respiratory capacity. (B) Mitochondrial membrane potential (ΔΨm) was assessed by flow cytometry at baseline, 1 h, and 24 h after oxidative stress. The bar graph summarizes the percentage of cells with polarized mitochondria. As controls, CCCP-treated cells were used as the positive control (loss of polarization), whereas unstained cells served as the negative control. All data are presented as mean ± SD (n = 3 from independent experiments). *p < 0.05, **p < 0.01, ***p < 0.001 vs. respective untreated controls; ###p < 0.001 vs. MSCs + H₂O₂.
Article Snippet: Mitochondrial respiratory activity was evaluated using an Enhanced
Techniques: Membrane, Activity Assay, Fluorescence, Flow Cytometry, Positive Control, Negative Control
Journal: Frontiers in Toxicology
Article Title: Combined exposure to emerging Fusarium mycotoxins produces concentration-dependent synergistic toxicity in Jurkat T cells
doi: 10.3389/ftox.2026.1924250
Figure Lengend Snippet: Effects of BEA, ENN B, and MON on intracellular reactive oxygen species (ROS) generation in human Jurkat T cells. Cells were exposed to increasing concentrations of BEA (0–7.24 μM), ENN B (0–17.88 μM), and MON (0–161.80 μM) for 24 h. ROS levels were measured using a Total ROS assay and expressed as relative fluorescence units (RFU). Data represent Mean ± SD of three independent biological experiments (n = 3), with individual experimental values shown as overlayed data points. Asterisks (*) indicate statistically significant differences compared to the control group ( p < 0.05).
Article Snippet: The
Techniques: ROS Assay, Fluorescence, Control
Journal: Frontiers in Toxicology
Article Title: Combined exposure to emerging Fusarium mycotoxins produces concentration-dependent synergistic toxicity in Jurkat T cells
doi: 10.3389/ftox.2026.1924250
Figure Lengend Snippet: Effects of BEA, ENN B, and MON, individually and in combination, on intracellular reactive oxygen species (ROS) generation in human Jurkat T cells. Cells were exposed to increasing concentrations of BEA, ENN B, and MON, alone or in binary and ternary combinations, for 24 h. ROS levels were measured using a Total ROS assay and expressed as relative fluorescence units (RFU) (A) BEA + ENN B (B) BEA + MON (C) ENN B+ MON, and (D) BEA + ENN B+ MON. Data represent Mean ± SD of three independent biological experiments (n = 3), with individual experimental values shown as overlayed data points. Asterisks (*) indicate statistically significant differences compared to the control group ( p < 0.05).
Article Snippet: The
Techniques: ROS Assay, Fluorescence, Control